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2026 , Vol. 46 >Issue 2: 9 - 17

DOI: https://doi.org/10.19638/j.issn1671-1114.20260202

生命科学

生物炭固定法对伯克霍尔德氏菌属FM-2菌株降解芴的影响

  • 罗泽天 ,
  • 马佳骏 ,
  • 高国辉 ,
  • 黄磊
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  • 天津理工大学 化学化工学院,天津 300384
罗泽天（2000—）,女,硕士研究生。
黄 磊（1980—）,男,教授,主要从事微生物降解以及胞外物质提取方面的研究.E-mail:huanglei@tjut.edu.cn.

收稿日期: 2024-12-23

  网络出版日期: 2026-06-03

基金资助

国家自然科学基金资助项目（42077212）

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Effect of biochar immobilization method on the degradation of fluorene by Burkholderia sp.FM-2

  • LUO Zetian ,
  • MA Jiajun ,
  • GAO Guohui ,
  • HUANG Lei
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  • School of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300384,China

Received date: 2024-12-23

  Online published: 2026-06-03

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摘要

为探究从石油污染土壤中筛选获得的 Burkholderia sp.FM-2 菌株对多环芳烃（PAHs）模式化合物芴的降解能力及代谢途径,并评估固定化生物菌剂的应用潜力,本研究通过改变降解环境条件开展相关实验。采用气相色谱法测定菌株的芴降解效率,使用气相色谱一质谱联用仪（GC-MS）检测芴降解中间产物,利用扫描电镜（SEM）观测生物炭表面微观结构及固定菌剂吸附情况,通过红外光谱分析（FTIR）探究生物炭和固定菌剂表面官能团的变化。结果显示:①该菌株在芴质量浓度100~500mg/L 范围内培养 3 d ,降解率均高于50%,在温度20~30℃、pH值7~9、盐度0~5g/L的条件下,接种 3 d 后300mg/L芴的降解率也保持在50%以上;②通过分析中间代谢产物推测其降解途径为经五元环单加氧反应生成 9-苞醇,进而转化为9-芴酮,最终生成邻苯二甲酸并持续代谢;③比较3种生物炭,最终选用秸秆生物炭通过吸附法制作固定菌剂,将芴的降解率提升至96.74%,该固定菌剂经 3 次循环利用后仍保持80%以上的降解率。

关键词： 伯克霍尔德氏菌属 FM-2; 多环芳烃; 芴; 生物降解; 生物炭; 固定菌剂

本文引用格式

罗泽天 , 马佳骏 , 高国辉 , 黄磊 . 生物炭固定法对伯克霍尔德氏菌属FM-2菌株降解芴的影响[J]. 天津师范大学学报（自然科学版）, 2026 , 46(2) : 9 -17 . DOI: 10.19638/j.issn1671-1114.20260202

Abstract

To explore the degrading capacity towards fluorene（a model compound of polycyclic aromatic hydrocarbons, PAHs）,and metabolic pathway of Burkholderia sp.FM-2,which is a strain isolated from petroleum-contaminated soil,and to evaluate the application potential of immobilized biological agents,a series of relevant experiments were conducted by al- tering degradation environmental conditions.Gas chromatography was used to determine the fluorene degradation efficiency of the strain,gas chromatography-mass spectrometry（GC-MS）was employed to detect the intermediate products of degra- dation,scanning electron microscopy（SEM）was utilized to observe the surface microstructure of biochar and the adsorp- tion of immobilized agents,and Fourier transform infrared spectroscopy（FTIR）was applied to investigate the changes in surface functional groups of biochar and immobilized agents.The results showed that:①When the strain was cultured for 3 d at a fluorene mass concentration range of 100~500mg/L,the degradation rate was all above 50% ;under the conditions of temperature 20~30℃, pH7-9,and salinity 0~5g/L,the degradation rate of 300mg/L fluorene remained above 50% after 3 d of inoculation.②By analyzing the intermediate metabolites,its degradation pathway was inferred as follows,i.e.9-fluo- renol is generated through the monohydroxylation reaction of the five-membered ring,which is then converted to 9 -fluo- renone,and finally o-phthalic acid is produced for continuous metabolism.③After comparing three types of biochar,straw biochar was selected to prepare immobilized agents by adsorption method,which increased the fluorene degradation rate to 96.74% ,and the immobilized agent still maintained a degradation rate of over 80% after three cycles of reuse.

Key words： Burkholderia sp.FM-2; PAHs; fluorene; biodegradation; biochar; immobilized bacterial agent

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