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2026 , Vol. 21 >Issue 1: 97 - 107

DOI: https://doi.org/10.19687/j.cnki.1673-7105.2026.01.011

碳循环与生态系统固碳

亚热带天然林土壤胞外酶及其根际效应对长期氮添加的响应

  • 黄证伊 ,
  • 程慧梓 ,
  • 陈铭 ,
  • 王小红 ,
  • 陈光水 ,
  • 姚晓东
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  • 1.福建师范大学地理科学学院、碳中和未来技术学院, 福州 350117;
    2.福建三明森林生态系统国家野外科学观测研究站, 福建三明 365002
*姚晓东(1992— ), 男, 福建宁德人, 副教授, 博士, 研究方向为地下生态学, xdyao@fjnu.edu.cn。

收稿日期: 2025-06-11

  网络出版日期: 2026-03-11

基金资助

福建省自然科学基金青年基金项目(2023J05118); 福建省中青年教师教育科研项目(科技类)(JAT220036); 国家自然科学基金青年基金项目(32301575); 国家自然科学基金区域创新发展联合基金项目(U25A20640)

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Responses of Rhizosphere and Bulk Soil Extracellular Enzyme Activities to Long-Term Nitrogen Addition in a Subtropical Castanopsis carlesii Natural Forest

  • HUANG Zhengyi ,
  • CHENG Huizi ,
  • CHEN Ming ,
  • WANG Xiaohong ,
  • CHEN Guangshui ,
  • YAO Xiaodong
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  • 1. School of Geographical Sciences, School of Carbon Neutrality Future Technology, Fujian Normal University, Fuzhou 350117, China;
    2. Sanming Forest Ecosystem National Observation and Research Station, Sanming 365002, Fujian, China

Received date: 2025-06-11

  Online published: 2026-03-11

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摘要

以亚热带米槠天然林为研究对象,依托长期模拟氮沉降观测样地,在生长季和非生长季测定氮添加下根际和非根际土壤的β-葡萄糖苷酶(βG)、亮氨酰氨基肽酶(LAP)、β-N-乙酰氨基葡萄糖苷酶(NAG)和酸性磷酸酶(AP)活性,并计算酶化学计量比(C/NEEA、C/PEEA、N/PEEA)、酶矢量长度和角度及根际效应值。结果发现：1)生长季的根际和非根际土壤βG活性较非生长季分别显著提升7.8倍和24.1倍,而AP活性则分别降低了41.0%、40.1%(P<0.05)。2)在生长季,低氮添加使根际土壤βG活性提升6.2%,C/PEEA与矢量长度显著增加,根际和非根际土壤酶矢量角度均显著降低(P<0.05)。在非生长季,低氮添加下根际土壤βG活性降低41.3%,C/PEEA、C/NEEA和酶矢量长度显著降低;高氮添加下,非根际土壤AP活性降低了44.9%,C/PEEA和N/PEEA也显著降低(P<0.05)。3)在非生长季,高氮添加加强了土壤βG、C/PEEA和N/PEEA的正根际效应,根际土壤微生物碳需求增加;酶矢量角度负根际效应加强,非根际土壤微生物磷限制加剧(P<0.05)。4)土壤速效磷含量是解释根际和非根际土壤酶活性随季节和氮添加变化的最主要因素。此外,土壤含水率和土壤速效氮养分也是影响土壤酶活性的重要因素。研究结果为氮沉降背景下亚热带天然林养分管理和可持续保护提供了重要的参考依据。

关键词： 长期氮添加; 土壤胞外酶; 根际效应; 酶化学计量

本文引用格式

黄证伊 , 程慧梓 , 陈铭 , 王小红 , 陈光水 , 姚晓东 . 亚热带天然林土壤胞外酶及其根际效应对长期氮添加的响应[J]. 亚热带资源与环境学报, 2026 , 21(1) : 97 -107 . DOI: 10.19687/j.cnki.1673-7105.2026.01.011

Abstract

This study investigated the responses of extracellular enzyme activities and their stoichiometric ratios to nitrogen(N) addition in rhizosphere and bulk soils of a subtropical Castanopsis carlesii natural forest.Based on a long-term simulated N deposition platform, the activities of β-glucosidase(βG),leucine aminopeptidase(LAP),β-N-acetylglucosaminidase(NAG), and acid phosphatase(AP) were measured during both growing and non-growing seasons.Enzyme stoichiometric ratios(C/NEEA,C/PEEA,N/PEEA),vector length and angle, and rhizosphere effects were calculated.Key findings include, 1) compared with the non-growing season, βG activities in rhizosphere and bulk soils increased significantly by 7.8 and 24.1 times respectively, during the growing season, whereas AP activities decreased by 41.0% and 40.1%(P<0.05).2) During the growing season, low-N addition increased rhizosphere soil βG activity by 6.2% and significantly increased C/PEEA and vector length, while enzyme vector angles decreased significantly in both rhizosphere and bulk soils(P<0.05).During the non-growing season, low-N addition decreased rhizosphere βG activity by 41.3% and significantly lowering C/PEEA,C/NEEA,and vector length. Under high-N addition, bulk soil AP activity declined by 44.9%, accompanied by significant decreases in C/PEEA and N/PEEA(P<0.05).3) During the non-growing season, high-N addition strengthened positive rhizosphere effects of βG,C/PEEA,N/PEEA,indicating enhanced carbon demand in rhizosphere microorganisms.Meanwhile, the strengthened negative rhizosphere effect on enzyme vector angle reflected aggravated phosphorus limitation in bulk soil microorganisms(P<0.05).4) Soil available phosphorus content was the most important factor explaining seasonal and nitrogen-induced variations in enzyme activities in both rhizosphere and bulk soils.Soil moisture and available nitrogen were also significant factors influencing soil enzyme activities.These results offer a scientific basis for nutrient management and sustainable protection of subtropical natural forests under the background of nitrogen deposition.

Key words： long-term nitrogen addition; soil extracellular enzyme; rhizosphere effect; enzyme chemical ratio

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