In order to investigate the mechanism of curcumin in reversing the resistance of non-small cell lung cancer to gefitinib,which is a kind of anticancer drug,and thus improve the efficacy of gefitinib,the A549 cell line was used as the experimental cell,and the gefitinib-resistant A549（A549／GR）cell line was successfully established.A549／GR cell line was divided into four treatment groups:control group,gefitinib group,curcumin group,and a combination group of gefitinib and curcumin.Cell Counting Kit-8（CCK-8）,colony formation,transwell,and flow cytometry were used to eval- uate the cell viability,colony formation capacity,migration,invasion,and apoptosis of A549 and A549／GR cells,respec- tively.The protein expression related to apoptosis,epithelial-mesenchymal transition（EMT）,and AKT signaling was studied using Western blot experiment,and the expression level of MET and AXL genes in A549 and A549／GR cells was de- tected by RT-PCR experiment.The results were as follows:①The combination of gefitinib and curcumin had synergistic effects,which enhanced chemosensitivity and cell apoptosis,and inhibited cell proliferation,colony formation,migration and invasion at the same time;②Western blot showed that curcumin significantly enhanced the ability of gefitinib to reverse EMT of A54／GR cells,induce apoptosis and inhibit AKT signaling;③RT-PCR experimental results showed that the up-reg- ulated MET and AXL signaling might be involved in the process of A549 cells resistant to gefitinib.In conclusion,the above results showed that curcumin could reverse the EMT of A549／GR cells through inhibiting the AKT signaling pathway,effec- tively enhance the sensitivity of cells to gefitinib,and promote the apoptosis of tumor cell.

To explore the degrading capacity towards fluorene（a model compound of polycyclic aromatic hydrocarbons, PAHs）,and metabolic pathway of Burkholderia sp.FM-2,which is a strain isolated from petroleum-contaminated soil,and to evaluate the application potential of immobilized biological agents,a series of relevant experiments were conducted by al- tering degradation environmental conditions.Gas chromatography was used to determine the fluorene degradation efficiency of the strain,gas chromatography-mass spectrometry（GC-MS）was employed to detect the intermediate products of degra- dation,scanning electron microscopy（SEM）was utilized to observe the surface microstructure of biochar and the adsorp- tion of immobilized agents,and Fourier transform infrared spectroscopy（FTIR）was applied to investigate the changes in surface functional groups of biochar and immobilized agents.The results showed that:①When the strain was cultured for 3 d at a fluorene mass concentration range of 100~500mg/L,the degradation rate was all above 50% ;under the conditions of temperature 20~30℃, pH7-9,and salinity 0~5g/L,the degradation rate of 300mg/L fluorene remained above 50% after 3 d of inoculation.②By analyzing the intermediate metabolites,its degradation pathway was inferred as follows,i.e.9-fluo- renol is generated through the monohydroxylation reaction of the five-membered ring,which is then converted to 9 -fluo- renone,and finally o-phthalic acid is produced for continuous metabolism.③After comparing three types of biochar,straw biochar was selected to prepare immobilized agents by adsorption method,which increased the fluorene degradation rate to 96.74% ,and the immobilized agent still maintained a degradation rate of over 80% after three cycles of reuse.

To clarify the cultural relics pests that damage the frescoes and clay sculptures in the Dule Temple in Jizhou Dis- trict of Tianjin,the pests occurring in the frescoes and clay sculptures in this temple were investigated.The classification and identification were conducted based on morphological characteristics and DNA barcoding technology.The results illustrated that:① The main pests of frescoes were Anthophora melanognatha,Trogoderma variabile,Tineola sp.,Uroctea sp.and Gibbium aequinoctiale;② The statistical analysis of all the holes of frescoes showed that the most pests inhabited in the holes with diameter less than 0.80 cm and depth less than 7.00 cm ;③ The depth of holes of Uroctea sp.had significant dif- ference with that of Tineola sp.,Trogoderma variabile,and A nthophora melanognatha (P<0.05),the depth of holes between Anthophora melanognatha and Tineola spp.also had significant differences (P<0.05),Tineola sp.had significant difference with Uroctea sp.and Anthophora melanognatha in the diameter of the holes (P<0.05) ,and there was also significant differ- ence in the diameter of the holes between Trogoderma variabile and Anthophora melanognatha (P<0.05);④The main pests of the clay sculpture were Trogoderma variabile,Uroctea sp.,Gibbium aequinoctiale,and Sphecidae sp.,and Anthophora melanognatha also nested in the chest of the Eleven-faced Avalokitesvara clay sculpture;⑤The related pests were eliminat- ed comprehensively using different methods and equipment,including insect-attracting method through colorful sticky boards,sugar-acetic acid-ethanol liquid,as well as pesticide spraying,injection and fumigation,etc.